Description
Description: This assay employs a two-site sandwich ELISA to quantitate YES1 in samples. An antibody specific for YES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.
The other 5 fragments could not be mapped either because hybridization signals were too weak or differentiation from mouse YES fragments was impossible. There was evidence for multiple copies of YES-related genes in the human genome, but only a single RNA species, 4.8 kb long, was found. At least 3 of the human YES gene copies had both introns and exons and 1 gene copy appeared to be a pseudogene